cop
18 maart 2007, 21:37
this sounds good :article of 13/03/2007 Researchers in Japan have just published a study where they succeeded in culturing dermal papilla cells for more than 30 passages (generations).
Usually when cells are cultured they lose their properties after only a few passages rendering them useless. Therefore being able to culture cells for more than 30 passages means that the amount of cells you can clone from a single source is significantly greater.
They were also able to induce the formation of new hair follicles by injecting these cells together with epidermal cells into mice.
Based on their study they concluded that fibroblast growth factor-2 is essential for the culturing of dermal papilla cells.
Long-Term Culture of Mouse Vibrissal Dermal Papilla Cells and De Novo Hair Follicle Induction
Tissue Eng. 2007 Mar 6
Osada A, Iwabuchi T, Kishimoto J, Hamazaki TS, Okochi H.
Department of Tissue Regeneration, Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo, Japan.
We have succeeded in culturing dermal papilla (DP) cells long term and developed new techniques that enhance their hair follicle-inducing efficiency in a patch assay.
The outgrowing DP cells from mouse vibrissae were markedly stimulated by 10% fetal bovine serum-Dulbecco’s modified essential medium that included fibroblast growth factor-2 (FGF-2). Moreover, the potency of proliferation was maintained during serial cultivations (more than 30 passages).
We combined these established DP cells with epidermal cells and implanted them subcutaneously into athymic mice to examine their hair follicle-inducing ability.
New hair follicles were induced by dissociated DP cells at earlier passages (under passage 4), but the cells from later passages could not induce follicles. We next aggregated the DP cells to form spheres and then injected them with epidermal cells. Unlike the dissociated DP cells, the spheres made from the later passaged cells (more than 10 passages) did induce new hair follicles.
We examined several genes specific for DP of anagen follicles and confirmed that their expression level was elevated in the spheres compared with their expression level in adherent DP cells.
These results suggest that FGF-2 is essential for dermal papilla cell culture and that sphere formation partially models the intact DP, resulting in hair follicle induction, even by later passaged cells.
Usually when cells are cultured they lose their properties after only a few passages rendering them useless. Therefore being able to culture cells for more than 30 passages means that the amount of cells you can clone from a single source is significantly greater.
They were also able to induce the formation of new hair follicles by injecting these cells together with epidermal cells into mice.
Based on their study they concluded that fibroblast growth factor-2 is essential for the culturing of dermal papilla cells.
Long-Term Culture of Mouse Vibrissal Dermal Papilla Cells and De Novo Hair Follicle Induction
Tissue Eng. 2007 Mar 6
Osada A, Iwabuchi T, Kishimoto J, Hamazaki TS, Okochi H.
Department of Tissue Regeneration, Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo, Japan.
We have succeeded in culturing dermal papilla (DP) cells long term and developed new techniques that enhance their hair follicle-inducing efficiency in a patch assay.
The outgrowing DP cells from mouse vibrissae were markedly stimulated by 10% fetal bovine serum-Dulbecco’s modified essential medium that included fibroblast growth factor-2 (FGF-2). Moreover, the potency of proliferation was maintained during serial cultivations (more than 30 passages).
We combined these established DP cells with epidermal cells and implanted them subcutaneously into athymic mice to examine their hair follicle-inducing ability.
New hair follicles were induced by dissociated DP cells at earlier passages (under passage 4), but the cells from later passages could not induce follicles. We next aggregated the DP cells to form spheres and then injected them with epidermal cells. Unlike the dissociated DP cells, the spheres made from the later passaged cells (more than 10 passages) did induce new hair follicles.
We examined several genes specific for DP of anagen follicles and confirmed that their expression level was elevated in the spheres compared with their expression level in adherent DP cells.
These results suggest that FGF-2 is essential for dermal papilla cell culture and that sphere formation partially models the intact DP, resulting in hair follicle induction, even by later passaged cells.